Abstract
Total parenteral nutrition (TPN) causes intrahepatic cholestasis and membrane phospholipid changes. Fatty acid (FA) composition of bile and hepatocyte phospholipid is influenced by dietary FA composition. We hypothesized that altering FA composition of i.v. lipid emulsions modifies 1) severity of TPN-induced cholestasis; 2) hepatocyte membrane composition and function; 3) bile flow and composition. Newborn piglets received either sow's milk, TPN with i.v. soybean oil or TPN with i.v. fish oil (FO). After 3 wk, basal and stimulated bile flow were measured after bolus injections of 20, 50, and 100 µmol/kg of taurocholate (TCA). Bile was analyzed for bile acids, cholesterol, phospholipids, and phospholipid-FA. Sinusoidal and canalicular membrane PL-FA, fluidity, and Na+/K+-ATPase were measured. Although the soybean oil-fed animals developed cholestasis, the FO and milk group had similar liver and serum bilirubin. Basal and stimulated bile flow rates were impaired in the soybean oil but not in the FO group. Hepatocyte membrane FA composition reflected dietary FA. Changes in sinusoidal and canalicular membrane fluidity and sinusoidal Na+/K+-ATPase activity did not explain the effect of FO on TPN-induced cholestasis. Intravenous FO reduces TPN-induced cholestasis by unknown mechanisms.
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Abbreviations
- BA:
-
bile acids
- BADF:
-
bile acid dependent component of bile flow
- BAIF:
-
bile acid independent component of bile flow
- C:
-
cholesterol
- FA:
-
fatty acids
- PL:
-
phospholipids
- TCA:
-
taurocholate
- TPN:
-
total parenteral nutrition
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Acknowledgements
The authors thank E. Paslawski and A. Wierzbicki for technical assistance and J. Minckler for secretarial contribution. MTE-conc trace elements were kindly donated by Lyphomed, Canada, calcium gluconate by Abbott, Canada, and MVI-Pediatric by Rhone-Poulenc Rorer, Canada. Drs. D. Duerksen and L. Gramlich were recipients of Glaxo Research Fellowships awarded through the Canadian Association of Gastroenterology.
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Supported by grants from the Medical Research Council of Canada, Alberta Heritage Foundation for Medical Research and Northern Alberta Children's Health Foundation.
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Van Aerde, J., Duerksen, D., Gramlich, L. et al. Intravenous Fish Oil Emulsion Attenuates Total Parenteral Nutrition-Induced Cholestasis in Newborn Piglets. Pediatr Res 45, 202–208 (1999). https://doi.org/10.1203/00006450-199902000-00008
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DOI: https://doi.org/10.1203/00006450-199902000-00008
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