Abstract
Lung injury in preterm neonates with respiratory failure has been attributed to persistent inflammation, which is likely to involve lung macrophages (LM). The study objective was to investigate LM during the first 8 d of life from preterm infants (n = 19), using term infants (n = 11) with respiratory failure as control subjects. LM percentages from mixed-cell suspensions produced from tracheobronchial lavage were calculated. A postnatal increase in the mean LM concentration was demonstrated within the preterm group (p = 0.01), which was greater in comparison to that from the term group (p < 0.01). Regression analyses were significant for direct relationships between LM concentrations and ex vivo lipopolysaccharide-induced tumor necrosis factor-α and IL-10 production (r = 0.93 and r = 0.63, respectively), establishing LM as the source of these cytokines. Comparative analyses demonstrated that the ability of preterm versus term LM to produce tumor necrosis factor-α was nearly identical; in contrast, a trend toward diminished levels of IL-10 expression in the preterm group was observed (p = 0.06). Thus, although studies have shown that LM precursors (i.e. cord blood monocytes) produce less tumor necrosis factor-α in preterm versus term infants, the present data strongly suggest that this relationship does not hold postnatally with respect to terminally differentiated LM in sick neonates. Overall, the data are consistent with a pro-versus antiinflammatory imbalance that may bear functional significance on the pathogenesis of chronic lung disease.
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Abbreviations
- LPS:
-
lipopolysaccharide
- LM:
-
lung macrophage(s)
- TBL:
-
tracheobronchial lavage
- TNF-α:
-
tumor necrosis factor-α
- CLD:
-
chronic lung disease
- DOL:
-
day of life
- RBC:
-
red blood cell
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Supported by the Hastings Foundation (HL60231).
Preliminary data for this study were presented at the 2000 Annual Meeting of the Society for Pediatric Research in Boston, MA, U.S.A.
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Blahnik, M., Ramanathan, R., Riley, C. et al. Lipopolysaccharide-Induced Tumor Necrosis Factor-α and IL-10 Production by Lung Macrophages from Preterm and Term Neonates. Pediatr Res 50, 726–731 (2001). https://doi.org/10.1203/00006450-200112000-00016
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DOI: https://doi.org/10.1203/00006450-200112000-00016
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