Abstract
The inorganic selenium compounds selenite and selenate are used for selenium fortification of infant formulas. However, information on absorption and retention of selenium from these compounds is lacking. The purpose of this study was therefore to determine apparent absorption and retention of selenium from selenate and selenite added to a milk-based infant formula in healthy infants. Labeled test meals were prepared by addition of 10 μg Se as 76Se-selenate or 74Se-selenite to 500 mL formula. The two batches of labeled formulas were fed as alternate feeds during the first day of the balance period, followed by unlabeled formula. Selenium isotopes were determined in feces collected for 72h after intake and in 3 consecutive 24h collections of urine. Mean apparent absorption was 97.1% for 76Se-selenate and 73.4% for 74Se-selenite; mean difference 23.7% (range: 13.8%–35.7%; SD 6.8%, p < 0.001). Mean urinary excretion (% of ingested dose) was 36.4% (76Se-selenate) and 9.7% (74Se-selenite); mean difference 26.7% (range: 13.9%–36.5%; SD 5.9%, p < 0.001). Mean apparent retention of selenium from 76Se-selenate and 74Se-selenite was not significantly different, 60.7% (76Se-selenate) versus 63.7% (for 74Se-selenite). The average difference was −3.01% (range: −14.0%–12.0%; SD 9.4%, p = 0.36). Although apparent selenium absorption and urinary excretion differed for selenite and selenate, selenium was equally well retained by infants from both selenium compounds. We therefore concluded that Se fortification of infant formulas with selenate or selenite can be expected to have similar impact on the selenium nutritional status of term infants.
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Abbreviations
- GC-MS:
-
gas chromatography mass spectrometry
- GPx:
-
glutathione peroxidase
- HGAAS:
-
hydride generation atomic absorption spectrometry
- ID:
-
iodothyronine deiodinase
- Se:
-
selenium
References
National Research Council 1989 Recommended Dietary Allowances, 10th ed. National Academy Press, Washington, DC, pp 217–224
World Health Organization 1996 Trace elements in human nutrition and health. WHO, Geneva, pp 105–122
Arner ESJ, Holmgren A 2000 Physiological functions of thioredoxin and thioredoxin reductase. Eur J Biochem 267: 6102–6109
Zhong L, Arner ESJ, Holmgren A 2000 Structure and mechanism of mammalian thioredoxin reductase: the active site is a redox-active selenothiol/selenosulfide formed from the conserved cysteine-selenocysteine sequence. Proc Natl Acad Sci USA 97: 5854–5859
Zachara BA 1993 Mammalian selenoproteins. J Trace Elem Health Dis 6: 137–151
Beck MA 1996 The role of nutrition in viral disease. J Nutr Biochem 7: 683–690
Clark LC, Combs GF, Turnbull BW 1996 Effects of selenium supplementation for cancer prevention in patients with carcinoma of the skin. J Am Med Assoc 276: 1957–1963
Litov R, Combs G 1991 Selenium in pediatric nutrition. Pediatrics 87: 339–351
Lombeck I, Kasperek K, Bonnermann B, Feinendegen LE, Bremer HJ 1978 Selenium content of human milk, cow's milk and cow's milk infant formulas. Eur J Pediatr 129: 139–145
Roekens E, Robberecht H, Van Caillie-Bertrand M, Deelstra H, Clara R 1985 Daily intake of selenium by bottle-fed infants in Belgium. Eur J Pediatr 144: 45–48
Robberecht H, Roekens E, Van Caillie-Bertrand M, Deelstra H, Clara R 1985 Longitudinal study of selenium content in human breast milk in Belgium. Acta Paediatr Scand 74: 254–258
Sanz Alaejos M, Diaz Romero C 1995 Selenium concentration in milk. Food Chem 52: 1–18
Kumpulainen J, Salmenperä, Siimes MA, Koivistoinen P, Lehto P, Perheenputa J 1987 Formula feeding results in lower selenium status than breast-feeding or selenium supplemented formula feeding: a longitudinal study. Am J Clin Nutr 45: 49–53
McGuire MK, Burgert SL, Milner JA, Glass L, Kummer R, Geering R, Boucek R, Picciano MF 1993 Selenium status of infants is influenced by supplementation of formula or maternal diets. Am J Clin Nutr 58: 643–648
Johnson CE, Smith AM, Chan GM, Moxeur-Mileur LJ 1993 Selenium status of term infants fed human milk or selenite-supplemented soy formula. J Pediatr 122: 739–741
Darlow B, Inder T, Sluis KB, Nuthall G, Mogridge N, Winterbourn CC 1995 Selenium status of New Zealand infants fed either a selenium supplemented or a standard formula. J Paediatr Child Health 31: 339–344
Smith AM, Chen LW, Thomas MR 1995 Selenate fortification improves selenium status of term infants fed soy formula. Am J Clin Nutr 61: 44–47
Solomons NW, Torun B, Janghorbani M, Christensen MJ, Young VR, Steinke FH 1986 Absorption of selenium from milk protein and isolated soy protein formulas in preschool children: studies using stable isotope tracer 74Se. J Pediatr Gastroenterol Nutr 5: 122–126
Ehrenkranz RA, Gettner PA, Nelli CM, Sherwonit EA, Williams JE, Ting BTG, Janghorbani M 1991 Selenium absorption and retention by very-low-birth- weight infants: studies with the extrinsic stable isotope tag 74Se. J Pediatr Gastroenterol Nutr 13: 125–133
Van Dael P, Van Cauwenbergh R, Deelstra H, Calomme M 1995 Determination of selenium in human serum by longitudinal Zeeman correction and flow injection hydride generation atomic absorption spectrometry. At Spectrosc 16: 251–257
Fomon SJ 1993 Procedures for collection of urine and feces and for metabolic studies. In: Fomon SJ (ed) Nutrition of Normal Infants. Mosby, St. Louis, MO, pp 459–464.
Van Dael P, Barclay D, Longet K, Metairon S, Fay LB 1998 Determination of selenium stable isotopes by gas chromatography-mass spectrometry with negative chemical ionisation. J Chromatogr B 715: 314–317
Belsten JL, Smith AJA 1995 European Community –FLAIR common assay for whole-blood glutathione peroxidase (GSH-Px). Results of an interlaboratory trial. Eur J Clin Nutr 49: 921–927
Turnlund JR, Keyes WR, Peiffer GL 1993 Isotope ratios of molybdenum determined by thermal ionisation mass spectrometry for stable isotope studies of molybdenum metabolism in humans. Anal Chem 65: 1717–1722
Heiberger RM 1989 Computation for the analysis of designed experiments. Wiley, New York.
Litov RE, Sickles VS, Chan GM, Hargett IR, Cordano A 1989 Selenium status in term infants fed human milk or infant formula with or without added selenium. Nutr Res 9: 585–596
Van Dael P, Davidsson L, Muñoz-Box R, Fay LB, Barclay D 2001 Selenium absorption and retention from a selenite or selenate-fortified milk-based formula in adult men measured by a stable isotope technique. Brit J Nutr 85: 157–163
Lönnerdal B, Glazier CE, Lien EL 1992 Selenium supplementation of infant formula: uptake and retention of various forms of selenium in suckling rats. J Nutr Biochem 3: 644–652
Wolffram S, Arduser F, Scharrer E 1985 In vivo intestinal absorption of selenate and selenite by rats. J Nutr 115: 454–459
Arduser F, Wolffram S, Scharrer E 1985 Active absorption of selenate by rat ileum. J Nutr 115: 1203–1208
Vendeland SC, Butler JA, Whanger PD 1992 Intestinal absorption of selenite, selenate, and selenomethionine in the rat. J Nutr Biochem 3: 359–365
Oster A, Prellwitz W 1990 The renal excretion of selenium. Biol Trace Elem Res 24: 119–146
Whanger PD 1998 Metabolism of selenium in humans. J Trace Elem Exp Med 11: 227–240
Thomson CD, Robinson MF 1986 Urinary and fecal excretions and absorption of a large supplement of selenium: superiority of selenate over selenite. Am J Clin Nutr 44: 659–663
Suzuki KT, Shiobara Y, Itoh M, Ohmichi M 1998 Selective uptake of selenite by red blood cells. Analyst 123: 63–67
Alfthan G, Aro A, Arvilommi H, Huttunen JK 1991 Selenium metabolism and platelet glutathione peroxidase activity in healthy Finnish men: effects of selenium yeast, selenite, and selenate. Am J Clin Nutr 53: 120–125
Smith AM, Chan GM, Moxeur-Mileur LJ, Johnson CE, Gardner BR 1991 Selenium status of preterm infants fed human milk, preterm formula, or selenite-supplemented preterm formula. J Pediatr 119: 429–433
Acknowledgements
The authors thank K. Longet, S. Metairon, and J. Clough for expert technical assistance, R. Hurrell (ETH Zürich, Switzerland) and P. Kastenmayerfor the advice, and R. Muñoz-Box and M. Baumgartner at the Nestlé Research Center for statistical evaluation. We also thank the nursing staff at the Fomon Infant Nutrition Unit for expert technical assistance and subjects and their parents for their participation.
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Van Dael, P., Davidsson, L., Ziegler, E. et al. Comparison of Selenite and Selenate Apparent Absorption and Retention in Infants Using Stable Isotope Methodology. Pediatr Res 51, 71–75 (2002). https://doi.org/10.1203/00006450-200201000-00013
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DOI: https://doi.org/10.1203/00006450-200201000-00013
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