Abstract
The influence of route of delivery, ambient temperature, maternal dexamethasone treatment, and postnatal age on plasma concentrations of leptin or leptin mRNA abundance in perirenal adipose tissue was examined from 6-h-old lambs, born vaginally or delivered by cesarean section into warm (30°C) or cool (15°C) ambient temperatures, and from cesarean section–delivered lambs whose mothers had been treated with dexamethasone beginning 2 d before parturition. The ontogeny of leptin during the first month of postnatal life was also examined. In lambs born into a cool ambient temperature, but not in those born to dexamethasone-treated mothers, leptin mRNA abundance decreased within 6 h of birth. Plasma concentrations of leptin decreased during the first 6 h of life, an adaptation delayed by cesarean section birth. After the first day of postnatal life, both plasma concentrations of leptin and its mRNA increased to peak at 7 d of age and were positively correlated with each other, as well as with whole-body and perirenal adipose tissue weights. A similar relationship was not observed after 7 d of age, as plasma leptin declined despite an increase in adipose tissue weight. In conclusion, route of delivery, ambient temperature, or maternal dexamethasone therefore delays the rate of leptin disappearance after birth. Concomitantly, leptin abundance was only associated with body and adipose tissue weights for 1 week after birth, which may be coincident with the onset of peak lactation and the time at which nutrient supply should no longer be limiting to the neonate.
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Abbreviations
- RT:
-
reverse transcription
- UCP1:
-
uncoupling protein-1
References
Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM 1994 Positional cloning of the mouse obese gene its human homologue. Nature 372: 425–432
Ahima RS, Flier JS 2000 Leptin. Annu Rev Physiol 62: 413–437
Friedman JM, Halaas JL 1998 Leptin the regulation of body weight in mammals. Nature 395: 763–770
Cetin I, Morpurga PS, Radaelli T, Taricco E, Cortelazzi D, Bellotti M, Pardi G, Beck-Peccoz P 2000 Fetal plasma leptin concentrations: relationship with different intrauterine growth patterns from 19 weeks to term. Pediatr Res 48: 646–651
Yuen BSJ, McMillen IC, Symonds ME, Owens JA 1999 Abundance of leptin messenger ribonucleic acid in fetal adipose tissue is related to fetal body weight. J Endocrinol 163: R1–R4
Hytinantti T, Koistinen HA 1999 Changes in leptin concentration during the early postnatal period: adjustment to extrauterine life?. Pediatr Res 45: 197–201
Masuzaki H, Ogawa Y, Sagawa N, Hosoda K, Matsu-moto T, Mise H, Nishimura H, Yoshimasa Y, Tanaka I, Mori T, Nakao K 1997 Non-adipose tissue production of leptin: leptin as a novel placenta-derived hormone in humans. Nat Med 3: 1029–1033
Clarke L, Bryant MJ, Lomax MA, Symonds ME 1997 Maternal manipulation of brown adipose tissue liver development in the ovine fetus during late gestation. Br J Nutr 77: 871–883
Clarke L, Heasman L, Firth K, Symonds ME 1997 Influence of route of delivery ambient temperature on thermoregulation in newborn lambs. Am J Physiol 272: R1931–R1939
Clarke L, Buss DS, Juniper DS, Lomax MA, Symonds ME 1997 Adipose tissue development during early postnatal life in ewe-reared lambs. Exp Physiol 82: 1015–1017
Casteilla L, Champigny O, Bouillaud F, Robelin J, Ricquier D 1989 Sequential changes in the expression of mitochondrial protein mRNA during the development of brown adipose tissue in bovine ovine species. Biochem J 257: 665–671
Fowden AL, Li J, Forhead AJ 1998 Glucocorticoids the preparation for life after birth: are there long-term consequences of the life insurance?. Proc Nutr Soc 57: 113–122
Shekhawat PS, Garland JS, Shivpuri C, Mick G, Sasidharan P, Pelz CJ, McCormick KL 1998 Neonatal cord blood leptin: its relationship to birth weight, body mass index, maternal diabetes, steroids. Pediatr Res 43: 338–413
Clarke L, Heasman L, Symonds ME 1998 Influence of maternal dexamethasone administration on thermoregulation in lambs delivered by caesarean section. J Endocrinol 156: 307–314
Lepercq J, Caucaz M, Lahlou N, Timsit J, Girard J, Auwerx J, de Mouzan SH 1998 Overexpression of placental leptin in diabetic pregnancy. Diabetes 47: 847–850
Matsuda J, Yokoto I, Iida M, Murakami T, Yamada M, Saijo T, Naito E, Ito M, Shima K, Kuroda Y 1999 Dynamic changes in serum leptin concentrations during the fetal neonatal periods. Pediatr Res 45: 71–75
Yuan C, Attele AS, Zhang L, Lynch JP, Xie J, Shi ZQ 2000 Leptin reduces body weight gain in neonatal rats. Pediatr Res 48: 380–383
Clarke L, Bird JA, Lomax MA, Symonds ME 1996 Effect of β3-adrenergic agonist (Zeneca D7114) on thermoregulation in near-term lambs delivered by cesarean section. Pediatr Res 40: 330–336
Delavaud C, Bocquier F, Chilliard Y, Keisler DH, Gertler A, Kann G 2000 Effect of sheep nutritional status body fatness on plasma leptin concentration assessed by a specific RIA. J Endocrinol 165: 519–526
Thomas L, Wallace JM, Aitken RP, Mercer JG, Trayhurn P, Hoggard N 2001 Circulating leptin during ovine pregnancy in relation to maternal nutrition, body composition pregnancy outcome. J Endocrinol 169: 465–476
Dessolin S, Schalling M, Champigny O, Lonnqvist F, Ailhaud G, Dani C, Ricquier D 1997 Leptin gene is expressed in rat brown adipose tissue at birth. FASEB J 11: 382–387
Arvaniti K, Ricquier D, Champigny O, Richard D 1998 Leptin corticosterone have opposite effects on food intake the expression of UCP1 mRNA in brown adipose tissue of lepob/lepobmice. Endocrinology 139: 4000–4003
Russel CD, Petersen RN, Rao SP, Ricci MR, Prasad A, Zhang Y, Brolin RE, Fried SK 1998 Leptin expression in adipose tissue from obese humans: depot specific regulation by insulin dexamethasone. Am J Physiol 275: E507–E515
Beran RK, Simons RW 2001 Cold-temperature induction of Escherichia coli polynucleotide phosphorylase occurs by reversal of its autoregulation. Mol Microbiol 39: 112–125
Trayhurn P, Duncan JS, Hoggrad N, Rayner DV 1998 Regulation of leptin production: a dominant role for the sympathetic nervous system?. Proc Nutr Soc 57: 413–419
Mostyn A, Keisler DH, Webb R, Stephenson T, Symonds ME 2001 The role of leptin in the transition from fetus to neonate. Proc Nutr Soc 60: 187–194
Rayner DV, Dalgliesh GD, Duncan JS, Hardie LJ, Hoggard N, Trayhurn P 1997 Postnatal development of the ob gene system: elevated leptin levels in suckling fa/fa rats. Am J Physiol 273: R446–R450
Vernon RG 1977 Development of perirenal adipose tissue in the neonatal lamb: effects of dietary safflower oil. Biol Neonate 32: 15–23
Darby CJ, Clarke L, Lomax MA, Symonds ME 1996 Brown adipose tissue liver development during early postnatal life in hand-reared ewe-reared lambs. Reprod Fertil Dev 8: 137–145
Symonds ME, Andrews DC, Johnson PJ 1989 The control of thermoregulation in the developing lamb during slow wave sleep. J Dev Physiol 11: 289–298
Symonds ME, Bryant MJ, Clarke L, Darby CJ, Lomax MA 1992 Effect of maternal cold exposure on brown adipose tissue thermogenesis in the neonatal lamb. J Physiol Lond 455: 487–502
McMillen IC, Phillips ID, Ross JT, Robinson JS, Owens JA 1995 Chronic stress—the key to parturition?. Reprod Fertil Dev 7: 197–206
Warnes K, Morris MJ, Symonds ME, Phillips ID, Owens JA, McMillen IC 1998 Effects of gestation, cortisol maternal undernutrition on hypothalamic neuropeptide Y mRNA levels in the sheep fetus. J Neuroendocrinol 10: 51–57
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Supported by a Ministry of Agriculture Fisheries and Food Postgraduate Studentship (J.D.) and a University of Nottingham Postgraduate Research Scholarship (A.M.).
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Bispham, J., Budge, H., Mostyn, A. et al. Ambient Temperature, Maternal Dexamethasone, and Postnatal Ontogeny of Leptin in the Neonatal Lamb. Pediatr Res 52, 85–90 (2002). https://doi.org/10.1203/00006450-200207000-00016
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DOI: https://doi.org/10.1203/00006450-200207000-00016
