Abstract
The channel proteins responsible for the cardiac transient outward K+ current (Ito) of human and rodent heart are composed, in part, of pore-forming Kv4.3 or Kv4.2 principal subunits. Recent reports implicate K+ channel interacting proteins (members of the neuronal Ca2+-binding protein family) as subunits of the Ito channel complex. We reported that another Ca2+-binding protein, frequenin [or neuronal calcium center protein-1 (NCS-1)], also functions as a Kv4 auxiliary subunit in the brain. By examining cardiac expression of NCS-1, the aim of this study was to examine the potential physiologic relevance of this protein as an additional regulator of cardiac Ito. Immunoblot analysis demonstrates NCS-1 protein to be expressed in adult mouse ventricle at levels comparable to that found in some brain regions. Cardiac NCS-1 protein expression levels are much higher in fetal and neonatal mouse hearts when compared with the adult. Immunocytochemical analysis of isolated neonatal mouse ventricular myocytes demonstrates co-localization of NCS-1 and Kv4.2 proteins at the sarcolemma. Given its high levels of expression in the heart, NCS-1 should be considered an important potential Kv4 regulatory subunit, particularly in the immature heart.
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Abbreviations
- Ito:
-
transient outward K+ current
- KChIPs:
-
K+ channel interacting proteins
- NCS-1:
-
neuronal calcium center protein-1 (also known as frequenin)
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Supported by the National Institutes of Health (Grant 1 R01 HL71058-01 to W.A.C.), the American Heart Association (Scientist Development Award 013019N to T.Y.N.), and by the Seventh District Association, Inc. E.S. was the recipient of a medical student research fellowship from the Society for Pediatric Research.
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Nakamura, T., Sturm, E., Pountney, D. et al. Developmental Expression of NCS-1 (Frequenin), a Regulator of Kv4 K+ Channels, in Mouse Heart. Pediatr Res 53, 554–557 (2003). https://doi.org/10.1203/01.PDR.0000057203.72435.C9
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DOI: https://doi.org/10.1203/01.PDR.0000057203.72435.C9
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