Abstract
We tested whether reoxygenation with 100% O2 for 5 min after experimental asphyxia in newborn piglets was as efficient as 100% O2 for 20 min compared with room air. Forty-one anesthetized piglets, 1–3 d old, were randomized to cerebral hypoxemia-ischemia-hypercapnia (HIH) or control (n = 5). HIH was achieved by ventilation with 8% O2, temporary occlusion of the common carotid arteries, and adding of CO2. After 25 min, reoxygenation-reperfusion was started with 100% O2 for 20 min (group 1, n = 12), or 21% 12), 100% O2 for 5 min (group 2, n = 12). All piglets were observed for 2 h. During O2 (group 3, n = reoxygenation-reperfusion, significantly higher blood pressure and more complete restoration of microcirculation (laser Doppler flow) in the cerebral cortex was found in both groups reoxygenated with 100% O2 compared with 21% O2 (regional cerebral blood flow ≥ 100%versus 70% of baseline, p = 0.04). Reoxygenation with 100% O2 for 5 min was as efficient as 20 min. Oxygen delivery in cortex was significantly higher in groups 1 and 2 compared with group 3 (p = 0.03), but there were no significant differences in cerebral metabolic rate for oxygen. In the striatum, no significant differences in flow or extracellular glutamate, glycerol, and lactate/pyruvate ratio were found between the groups. In conclusion, after experimental asphyxia, newborn piglets can be reoxygenated as efficiently with 100% O2 for only 5 min as 100% O2 for 20 min compared with room air.
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Abbreviations
- BE:
-
base excess
- CaO2:
-
arterial O2 content
- CvssO2:
-
sagittal sinus O2 content
- CBF:
-
cerebral blood flow
- CMRO2:
-
metabolic rate for oxygen
- DO2:
-
oxygen delivery
- HIH:
-
hypoxemia-ischemia-hypercapnia
- MABP:
-
mean arterial blood pressure
- OER:
-
oxygen extraction ratio
- PaCO2:
-
arterial CO2 tension
- PaO2:
-
arterial O2 tension
- ROS:
-
reactive oxygen species
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Acknowledgements
The authors thank the staff at the Institute for Experimental Medical Research, Ullevaal University Hospital, for their skilled technical assistance, enthusiasm, and support.
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Supported in part by grants from the Medical Faculty, University of Oslo; the Norwegian Society of Anaesthesiology; and Rolf Geir Gjertsen's Foundation. A.B.S. is a research fellow with the Norwegian Council on Cardiovascular Diseases.
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Solås, AB., Munkeby, B. & Saugstad, O. Comparison of Short- and Long-Duration Oxygen Treatment after Cerebral Asphyxia in Newborn Piglets. Pediatr Res 56, 125–131 (2004). https://doi.org/10.1203/01.PDR.0000128978.90201.1D
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DOI: https://doi.org/10.1203/01.PDR.0000128978.90201.1D
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