Abstract
The present study assessed the effects of dietary restriction on tibial and vertebral mechanical and geometrical properties in 2-mo-old male Wistar rats. Two-month-old male Wistar rats were randomized to the ad libitum (n = 8) or the 35% diet-restricted (DR) feeding group (n = 9) for 5 mo. Tibiae and L6 vertebrae were dissected out for microcomputed tomography (μCT) scanning and subsequently fractured in biomechanical testing to determine geometrical and mechanical properties. The DR group had significantly lower mean tibial length, mass, area, and cross-sectional moment of inertia, as well as vertebral energy to maximal load. After adjustment for body mass, however, DR tibial mean maximal load and stiffness, and DR vertebral area, height, volume, and maximal load were significantly greater, relative to ad libitum means. No significant differences were found between the DR and ad libitum mineral ash fractions. Because the material properties of the tibiae between the two groups were not significantly different, presumably the material integrity of the bones was not adversely affected as a consequence of DR. The similar material characteristics were consistent with mineral ash fractions that were not different between the two groups. Vertebral maximal load and stiffness were not significant between the DR and ad libitum animals. Importantly, we show that a level of dietary restriction (35%) that is less severe than many studies (40%), and without micronutrient compensation does not adversely affect tibial and vertebral mechanical properties in young growing male rats when normalized for body mass.
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Abbreviations
- BMD:
-
bone mineral density
- DR:
-
diet-restricted
- L6:
-
6th lumbar
- μCT:
-
microcomputed tomography
References
Weindruch R, Sohal RS 1997 Seminars in medicine of the Beth Israel Deaconess Medical Center. Caloric intake and aging. N Engl J Med 337: 86–994
McCay CM, Crowell MF, Maynard LA 1935 The effect of retarded growth upon the length of life span and upon the ultimate body size. J Nutr 10: 3–79
McCay CM, Maynard LA, Sperling C, Barnes LL 1939 Retarded growth, life span, ultimate body size and age changes in the albino rat after feeding diets restricted in calories. J Nutr 18: 1–13
Ferguson VL, Greenberg AR, Bateman TA, Ayers RA, Simske SJ 1999 The effects of age and dietary restriction without nutritional supplementation on whole bone structural properties in C57BL/6J mice. Biomed Sci Instrum 35: 5–91
LaMothe JM, Hepple RT, Zernicke RF 2003 Selected contribution: Bone adaptation with aging and long-term caloric restriction in Fischer 344 x Brown-Norway F1-hybrid rats. J Appl Physiol 95: 739–1745
Lee CJ, Panemangalore M, Wilson K 1986 Effect of dietary energy restriction on bone mineral content of mature rats. Nutr Res 6: 1–59
Talbott SM, Rothkopf MM, Shapses SA 1998 Dietary restriction of energy and calcium alters bone turnover and density in younger and older female rats. J Nutr 128: 40–645
Talbott SM, Cifuentes M, Dunn MG, Shapses SA 2001 Energy restriction reduces bone density and biomechanical properties in aged female rats. J Nutr 131: 382–2387
Lane MA, Raznik AZ, Tilmont EM, Lanir A, Ball SS, Read V, Ingram DK, Cutler RG, Roth GS 1995 Aging and food restriction alter some indices of bone metabolism in male rhesus monkeys (Macaca mulatta). J Nutr 125: 600–1610
Lee CJ, Wang C, Panemangalore M 1993 Effect of continuous or cyclic restriction of energy intake on bone mineral content of oophorohysterectomized rats. Nutr Res 13: 63–573
Wronski TJ, Schenck PA, Clintron M, Walsh CC 1987 Effect of body weight on osteopenia in ovariectomized rats. Calcif Tissue Int 40: 55–159
Blain H, Vuillemin A, Guillemin F, Durant R, Hanesse B, de Talance N, Doucet B, Jeandel C 2002 Serum leptin level is a predictor of bone mineral density in postmenopausal women. J Clin Endocrinol Metab 87: 030–1035
McGuigan FE, Murray L, Gallagher A, Davey-Smith G, Neville CE, Van't Hof R, Boreham C, Ralston SH 2002 Genetic and environmental determinants of peak bone mass in young men and women. J Bone Miner Res 17: 273–1279
Turturro A, Hart RW 1991 Longevity-assurance mechanisms and caloric restriction. Ann N Y Acad Sci 621: 63–372
Heilbronn LK, Ravussin E 2003 Calorie restriction and aging: review of the literature and implications for studies in humans. Am J Clin Nutr 78: 61–369
Masoro EJ 2000 Caloric restriction and aging: an update. Exp Gerontol 35: 99–305
Chowdhury P, Rayford PL 2001 Effect of food restriction on plasma cholecystokinin levels and exocrine pancreatic function in rats. Ann Clin Lab Sci 31: 76–382
Brochmann EJ, Duarte ME, Zaidi HA, Murray SS 2003 Effects of dietary restriction on total body, femoral, and vertebral bone in SENCAR, C57BL/6, and DBA/2 mice. Metabolism 52: 265–1273
Diniz YS, Cicogna AC, Padovani CR, Silva MD, Faine LA, Galhardi CM, Rodrigues HG, Novelli EL 2003 Dietary restriction and fibre supplementation: oxidative stress and metabolic shifting for cardiac health. Can J Physiol Pharmacol 81: 042–1048
Korczynska J, Stelmanska E, Swierczynski J 2003 Differential effect of long-term food restriction on fatty acid synthase and leptin gene expression in rat white adipose tissue. Horm Metab Res 35: 93–597
Pelker RR, Friedlaender GE, Markham TC, Panjabi MM, Moen CJ 1984 Effects of freezing and freeze-drying on the biomechanical properties of rat bone. J Orthop Res 1: 05–411
Peng Z, Tuukkanen J, Zhang H, Jamsa T, Vaananen HK 1994 The mechanical strength of bone in different rat models of experimental osteoporosis. Bone 15: 23–532
Akhter MP, Iwaniec UT, Covey MA, Cullen DM, Kimmel DB, Becker R 2000 Genetic variation in bone density, histomorphometry, and strength in mice. Calcif Tissue Int 67: 37–344
Linde F, Hvid I 1989 The effect of constraint on the mechanical behavior of trabecular bone specimens. J Biomech 22: 85–490
Judex S, Zernicke RF 2000 High-impact exercise and growing bone: relation between high strain rates and enhanced bone formation. J Appl Physiol 88: 183–2191
Gatti D, Sartori E, Braga V, Corallo F, Rossini M, Adami S 2001 Radial bending breaking resistance derived by densitometric evaluation predicts femoral neck fracture. Osteoporos Int 12: 64–869
Zernicke RF, Salem GJ, Barnard RJ, Schramm E 1995 Long-term, high-fat-sucrose diet alters rat femoral neck and vertebral morphology, bone mineral content, and mechanical properties. Bone 16: 5–31
Banu J, Orhii PB, Okafor MC, Wang L, Kalu DN 2001 Analysis of the effects of growth hormone, exercise and food restriction on cancellous bone in different bone sites in middle-aged female rats. Mech Ageing Dev 122: 49–864
Ndiaye B, Cournot G, Pelissier MA, Debray OW, Lemonnier D 1995 Rat serum osteocalcin concentration is decreased by restriction of energy intake. J Nutr 125: 283–1290
Egger CD, Muhlbauer RC, Felix R, Delmas PD, Marks SC, Fleisch H 1994 Evaluation of urinary pyridinium crosslink excretion as a marker of bone resorption in the rat. J Bone Miner Res 9: 211–1219
Kimmel DB 1994 In vivo animal models in osteoporosis research. In: Marcus R (ed) Osteoporosis. Blackwell Scientific Publications, Boston, pp 354–382
Kalu DN, Hardin RR, Cockerham R, Yu BP, Norling BK, Egan JW 1984 Lifelong food restriction prevents senile osteopenia and hyperparathyroidism in F344 rats. Mech Ageing Dev 26: 103–112
Sato M, Vahle J, Schmidt A, Westmore M, Smith S, Rowley E, Ma LY 2002 Abnormal bone architecture and biomechanical properties with near-lifetime treatment of rats with PTH. Endocrinology 143: 230–3242
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Supported in part by Natural Sciences and Engineering Research Council, Canadian Institutes of Health Research, Alberta Heritage Foundation for Medical Research, and the Margaret Gunn Endowment for Animal Research.
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Lambert, J., Lamothe, J., Zernicke, R. et al. Dietary Restriction Does Not Adversely Affect Bone Geometry and Mechanics in Rapidly Growing Male Wistar Rats. Pediatr Res 57, 227–231 (2005). https://doi.org/10.1203/01.PDR.0000148715.61869.4E
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DOI: https://doi.org/10.1203/01.PDR.0000148715.61869.4E
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