Abstract
Infants develop hypertrophic cardiomyopathy in ≈30% of diabetic pregnancies. We have characterized the effects of glucose on voltage-gated T-type Ca2+ channels and intracellular free calcium concentration, [Ca2+]i in neonatal rat cardiomyocytes. We found that T-type Ca2+ channel current density increased significantly in primary culture neonatal cardiac myocytes that were treated with 25 mM glucose for 48 h when compared with those that were treated with 5 mM glucose. High-glucose treatment also caused a higher Ca2+ influx elicited by 50 mM KCl in the myocytes. KCl-induced Ca2+ influx was attenuated when nickel was present. Real-time PCR studies demonstrated that mRNA levels of both α1G (Cav3.1) and α1H (Cav3.2) T-type Ca2+ channels were elevated after high-glucose treatment. High-glucose also significantly increased ventricular cell proliferation as well as the proportion of cells in the S-phase of the cell cycle; both effects were reversed by nickel or mibefradil. These results indicate that high glucose causes a rise in [Ca2+]i in neonatal cardiac myocytes by a mechanism that is associated with the regulation of the T-type Ca2+ channel activity.
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Abbreviations
- [Ca2+]i:
-
cytoplasmic free Ca2+ concentration
- FBS:
-
fetal bovine serum
- IDM:
-
infants of diabetic mothers
- RT-PCR:
-
reverse transcriptase–PCR
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This study was supported by a research-in-aid award of the American Heart Association to M.L.
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Li, M., Zhang, M., Huang, L. et al. T-Type Ca2+ Channels Are Involved in High Glucose–Induced Rat Neonatal Cardiomyocyte Proliferation. Pediatr Res 57, 550–556 (2005). https://doi.org/10.1203/01.PDR.0000155756.89681.3C
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DOI: https://doi.org/10.1203/01.PDR.0000155756.89681.3C
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