Abstract
C3H/HeJ mice develop an increase in terminal air space area detectable by postnatal d 14 that persists into adulthood compared with strain-matched controls (C3H/SnJ, C3H/OuJ). Morphometric quantification revealed a 50% increase in terminal air space area by postnatal d 14 and a 2.3-fold increase by 2 mo of age in C3H/HeJ mice. Bacteriologic cultures obtained from the left lung on postnatal d 7 revealed >100 colony-forming units (CFU)/left lung of predominantly Gram-negative bacteria (GNB) (Escherichia coli and Proteus mirabilis) in 13 of the 14 C3H/HeJ mice compared with 0 of 12 controls demonstrating colonization of the developing lung in C3H/HeJ mice. An approximately threefold increase in macrophages from bronchoalveolar lavage, threefold increases in matrix metalloproteinase 12 (MMP-12) mRNA and protein levels and elevated levels of proinflammatory cytokines monocyte chemoattractant protein (MCP-1) and keratinocyte-derived cytokine (KC) were also found. P. mirabilis obtained from lung cultures in C3H/HeJ mice induced nuclear factor-κB (NF-κB) activation in human embryonic kidney 293 (HEK 293) cells transfected with TLR5. In C3H/HeJ mice lacking TLR4 signaling, bacterial colonization is associated with chronic inflammation and permanent changes in lung morphology.
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Abbreviations
- HEK 293:
-
human embryonic kidney 293
- KC:
-
keratinocyte-derived cytokine
- LPS:
-
lipopolysaccharide
- MCP-1:
-
monocyte chemoattractant protein
- MMP-12:
-
matrix metalloproteinase 12
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Sampath, V., Davis, K., Senft, A. et al. Altered Postnatal Lung Development in C3H/HeJ Mice. Pediatr Res 60, 663–668 (2006). https://doi.org/10.1203/01.pdr.0000246071.50268.51
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DOI: https://doi.org/10.1203/01.pdr.0000246071.50268.51
