Abstract
Fundamental questions remain about the optimal temperature, duration, and mode of delivery that provide the best striatal neuroprotection from hypothermia after perinatal hypoxia-ischemia. This study used stereological methods to investigate whether a mild (i.e. 2°C) or a moderate (5°C) decrease in whole body temperature, for 6 h immediately postinsult, was neuroprotective for striatal medium-spiny neurons after perinatal hypoxia-ischemia in the rat. This study also investigated whether moderate hypothermia had any effect on normal striatal development. Hypoxia-ischemia or sham hypoxia-ischemia was induced on postnatal day (PN) 7. Pups were kept either normothermic, mildly hypothermic, or moderately hypothermic for 6 h immediately postinsult. The absolute number of striatal medium-spiny neurons was calculated using modern stereological methods. There was no significant difference in the absolute number of medium-spiny neurons in the right striatum after either mild hypothermia or moderate hypothermia. There was also no significant difference in the absolute number of medium-spiny neurons between the control normothermic and the control moderately hypothermic pups. The latter results suggest that moderate hypothermia for 6 h immediately postinsult may be a safe treatment for striatal medium-spiny neurons. Yet, neither mild nor moderate hypothermia alone for 6 h immediately posthypoxia-ischemia is neuroprotective for striatal medium-spiny neurons.
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Abbreviations
- CE:
-
coefficient of error
- H/I:
-
hypoxic/ischemic
- N:
-
total (i.e. absolute) number of neurons
- NV:
-
neuronal density
- PN7:
-
postnatal day 7
- PN14:
-
postnatal day 14
- Vref:
-
total reference volume
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Covey, M., Oorschot, D. Effect of Hypothermic Post-Treatment on Hypoxic-Ischemic Striatal Injury, and Normal Striatal Development, in Neonatal Rats: A Stereological Study. Pediatr Res 62, 646–651 (2007). https://doi.org/10.1203/PDR.0b013e318157d1fe
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DOI: https://doi.org/10.1203/PDR.0b013e318157d1fe
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