Abstract
To examine whether the functional and morphologic conversion of the neonatal ascending thin limb (ATL) of Henle's loop is related to gestational length, we evaluated the transepithelial voltages (Vts) of ATLs in perinatal mouse, hamster, rabbit, and rat kidneys. In isolated microperfused tubule preparations, Vts of neonatal ATLs were 23.8 ± 1.4 in mouse, 25.7 ± 2.2 in hamster, and 18.2 ± 1.6 mV in rabbit. The influence of gestational length on the Vts and rat Na-K-Cl cotransporter (rNKCC2) expression pattern was also examined in perinatal rats subjected to a prolonged gestation due to either a daily s.c. injection of 5 mg progesterone or ligation of the extremities of the uterine horn. Vts of d 3 neonates were 2.9 ± 1.0 (p < 0.0001 versus d 0); Vts of d 23 fetuses subjected to ligation were 4.9 ± 0.8 (p < 0.005 versus d 0); and Vts of d 23 fetuses given progesterone were 3.4 ± 1.7 mV (p < 0.001 versus d 0). rNKCC2 expression tended to disappear in the renal papillae of d 23 fetuses. Our data demonstrate that the perinatal conversion of the ATL is a phenomenon commonly observed among rodents; furthermore, it is dependent on the gestational length, but unrelated to the birth process.
Similar content being viewed by others
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
Abbreviations
- ATL:
-
ascending thin limb
- rNKCC2:
-
rat Na-K-Cl cotransporter
- TAL:
-
thick ascending limb
- Vt:
-
transepithelial voltage
References
Spitzer A, Schwartz G 1992 The kidney during development. In: Windhager E (ed) Handbook of Physiology: Renal Physiology. Oxford University Press, New York, pp 475–544
Edwards BR, Mendel DB, LaRochelle FT Jr Stern P, Valtin H 1982 Postnatal development of urinary concentrating ability in rats: changes in renal anatomy and neurohypophysial hormones. In: Spitzer A (ed) The Kidney During Development: Morphology and Function. Masson, New York, pp 233–240.
Hamburger O 1890 Uber dir Entwicklung der Saugetierniere. Arch Anat Physiol suppl: 15–51
Kim J, Lee GS, Tisher CC, Madsen KM 1996 Role of apoptosis in development of the ascending thin limb of the loop of Henle in rat kidney. Am J Physiol 271: F831–F845
Liu W, Morimoto T, Kondo Y, Iinuma K, Uchida S, Imai M 2001 Avian-type” renal medullary tubule organization causes immaturity of urine-concentrating ability in neonates. Kidney Int 60: 680–693
Nishimura H, Imai M, Ogawa M 1986 Diluting segment in avian kidney. I. Characterization of transepithelial voltages. Am J Physiol 250: R333–R340
Nishimura H, Koseki C, Imai M, Braun EJ 1989 Sodium chloride and water transport in the thin descending limb of Henle of the quail. Am J Physiol 257: F994–F1002
Nishimura H, Koseki C, Patel TB 1996 Water transport in collecting ducts of Japanese quail. Am J Physiol 271: R1535–R1543
Nishimura H, Fan Z 2002 Sodium and water transport and urine concentration in avian kidney. Symp Soc Exp Biol
Thliveris JA 1976 Fine structure of the placental labyrinth in the rat at term and during prolonged gestation. Virchows Arch B Cell Pathol 21: 169–178
Faridy EE 1981 Fetal lung development in surgically induced prolonged gestation. Respir Physiol 45: 153–166
Burg M, Grantham J, Abramow M, Orloff J 1966 Preparation and study of fragments of single rabbit nephrons. Am J Physiol 210: 1293–1298
Kondo Y, Fromter E 1987 Axial heterogeneity of sodium-bicarbonate cotransport in proximal straight tubule of rabbit kidney. Pflugers Arch 410: 481–486
Kaplan MR, Plotkin MD, Lee WS, Xu ZC, Lytton J, Hebert SC 1996 Apical localization of the Na-K-Cl cotransporter, rBSC1, on rat thick ascending limbs. Kidney Int 49: 40–47
Imai M, Kusano E 1982 Effects of arginine vasopressin on the thin ascending limb of Henle's loop of hamsters. Am J Physiol 243: F167–F172
Haas M, Forbush B 3rd 1998 The Na-K-Cl cotransporters. J Bioenerg Biomembr 30: 161–172
Farman N 1996 Na,K-pump expression and distribution in the nephron. Miner Electrolyte Metab 22: 272–278
Satlin LM 1994 Postnatal maturation of potassium transport in rabbit cortical collecting duct. Am J Physiol 266: F57–F65
Satlin LM, Sheng S, Woda CB, Kleyman TR 2001 Epithelial Na+ channels are regulated by flow. Am J Physiol Renal Physiol 280: F1010–F1018
Baum M, Biemesderfer D, Gentry D, Aronson PS 1995 Ontogeny of rabbit renal cortical NHE3 and NHE1: effect of glucocorticoids. Am J Physiol 268: F815–F820
Ballard PL 1979 Glucocorticoids and differentiation. In: Baxter JD, Rousseau GG (eds) Monographs on Endocrinology Glucocorticoid Hormone Action. Springer-Verlag, New York, pp 493–515
Greengard O 1970 The developmental formation of enzymes in rat liver. In: Litwack G (ed) Biochemical Actions of Hormones. Academic Press, New York, pp 53–87
Henning SJ 1981 Postnatal development: coordination of feeding, digestion, and metabolism. Am J Physiol 241: G199–G214
Acknowledgements
The authors thank Naoko Sato for her technical assistance.
Author information
Authors and Affiliations
Corresponding author
Additional information
This work was supported in part by a grant-in-aid from the Ministry of Education, Science, and Culture in Japan and by a research grant 02C6 from the Salt Science Research Foundation in Japan.
Rights and permissions
About this article
Cite this article
Nishino, M., Morimoto, T., Nishio, T. et al. Gestational Length Affects a Change in the Transepithelial Voltage and the rNKCC2 Expression Pattern in the Ascending Thin Limb of Henle's Loop. Pediatr Res 61, 171–175 (2007). https://doi.org/10.1203/pdr.0b013e31802d7791
Received:
Accepted:
Issue date:
DOI: https://doi.org/10.1203/pdr.0b013e31802d7791
