Abstract
Using an 11.7-Tesla magnetic resonance imaging (MRI) scanner in 10-d-old rat pups we report on the evolution of injury over 28 d in a model of neonatal stroke (transient filament middle cerebral artery occlusion, tfMCAO) and a model of hypoxic-ischemic injury (Rice-Vannucci model, RVM). In both models, diffusion-weighted imaging (DWI) was more sensitive in the early detection of ischemia than T2-weighted imaging (T2WI). Injury volumes in both models were greater on d 1 for DWI and d 3 for T2WI, decreased over time and by d 28 T2WI injury volumes (tfMCAO 10.3% of ipsilateral hemisphere; RVM 23.9%) were definable. The distribution of injury with tfMCAO was confined to the vascular territory of the middle cerebral artery and a definable core and penumbra evolved over time. Ischemic injury in the RVM was more generalized and greater in cortical regions. Contralateral hemispheric involvement was only observed in the RVM. Our findings demonstrate that high-field MRI over extended periods of time is possible in a small animal model of neonatal brain injury and that the tfMCAO model should be used for studies of neonatal stroke and that the RVM does not reflect the vascular distribution of injury seen with focal ischemia.
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Abbreviations
- DWI:
-
diffusion-weighted imaging
- FOV:
-
field of view
- MCA:
-
middle cerebral artery
- RVM:
-
Rice-Vannucci model
- tfMCAO:
-
transient middle cerebral artery occlusion
- SHR:
-
spontaneously hypertensive rat
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Acknowledgements
The authors thank Dr. Yata Kenichiro and Dr. John Zhang for their assistance in the RVM experiments and Drs. David Michelson and Brenda Bartnik for their review of the manuscript.
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Supported by the Pediatric Research Fund, Department of Pediatrics, Loma Linda University School of Medicine, grant number OSR# 83018.
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Ashwal, S., Tone, B., Tian, H. et al. Comparison of Two Neonatal Ischemic Injury Models Using Magnetic Resonance Imaging. Pediatr Res 61, 9–14 (2007). https://doi.org/10.1203/01.pdr.0000251612.16069.4b
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DOI: https://doi.org/10.1203/01.pdr.0000251612.16069.4b
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