Abstract
This longitudinal, prospective study sought to establish whether pediatric Crohn's disease (CD) and ulcerative colitis (UC) are associated with increased levels of cytogenetic damage and whether folate supplementation in combination with other treatments mitigates cytogenetic damage in children with inflammatory bowel disease (IBD). After a 1-mo treatment and folate supplementation, all clinical tests in CD (n = 24) and UC (n = 17) patients improved. Patients with CD were comparable in the cytogenetic response with controls (n = 28) assessed by micronucleus (MN) assay, but both groups differed from the UC group. While the MN frequency in epithelial cells slightly decreased from first to second observations in CD patients (p = 0.05) and controls (p = 0.11), an increase was observed in UC patients (p = 0.001). Similar changes were observed in blood lymphocytes resulting in significantly higher levels of the MNs and chromosome bridges in UC patients. These preliminary findings of a difference in chromosome damage between pediatric UC patients compared with CD patients and healthy controls warrant confirmation and expansion to determine (1) the role of cytogenetic damage in the pathogenesis of these diseases, (2) relative contribution of treatment and folate supplementation, and (3) potential links to the eventual development of cancer in some patients.
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Abbreviations
- BNs:
-
binucleated lymphocytes
- CD:
-
Crohn's disease
- IBD:
-
inflammatory bowel disease
- GI:
-
gastrointestinal
- NBs:
-
nucleoplasmic bridges
- RI:
-
replicative index
- UC:
-
ulcerative colitis
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Acknowledgements
The authors thank R. Momi, and C. Tran for their help with the scoring and J. Cooper, J. Madden, and N. Sweeters for assistance with subject recruitment. Dr. M. Smith's support of this project is appreciated. This study would not be possible without the cooperation of our patients and control subjects.
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Supported by grants from the Packard Foundation, the Crohn's and Colitis Foundation of America, and NIH (M01 RR01271 and K24 DK60617). The contents of this article are solely the responsibility of the authors and do not necessarily represent official views of the Packard Foundation, the Crohn's and Colitis Foundation of America, and NIH.
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Holland, N., Harmatz, P., Golden, D. et al. Cytogenetic Damage in Blood Lymphocytes and Exfoliated Epithelial Cells of Children With Inflammatory Bowel Disease. Pediatr Res 61, 209–214 (2007). https://doi.org/10.1203/pdr.0b013e31802d77c7
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DOI: https://doi.org/10.1203/pdr.0b013e31802d77c7
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