Abstract
Neurofibromatosis type 1 (NF1) is a hereditary disease caused by mutations of the NF1 gene at 17q11.2. Loss of the NF1 gene product in Schwann cells leads to the development of benign nerve sheath tumors. These neurofibromas may occur at any time but tend to arise during periods of hormonal imbalance, suggesting that hormones influence neurofibroma growth. As steroid hormone levels rise during these times, we hypothesized that progesterone has proliferative effects on neurofibroma-derived Schwann cells. We chose specific medium conditions for selective proliferation of NF (+/−) and NF (−/−) cells from human neurofibromas. Genetic characterization was not performed, but former works have shown that under the conditions used (+/−) and (−/−) cells can be selected. Different progesterone concentrations were added at different days with BrdU-staining was performed to investigate proliferation rates and DAB-staining to identify a progesterone receptor. We could demonstrate that Schwann cells from human neurofibromas express progesterone receptors. These cells show elevated proliferation rates (highest in NF(−/−) cells) under progesterone, whereas normal human Schwann cells were not affected. These data suggest that progesterone plays an important role in the development of neurofibromas in NF1.
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Abbreviations
- NF1:
-
neurofibromatosis type 1
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Acknowledgements
We thank Dr. Conxi Lázaro and Dr. Eduard Serra, Medical and Molecular Genetics Center-IRO, Hospital Duran i Reynals, L'Hospitalet de Llobregat, Barcelona/Spain for continuous intellectual and molecular genetic support as well as critical reading and comments on the manuscript. We thank Murray Hartley, Great Britain, for proof reading our manuscript.
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This work was supported by Deutsche Krebshilfe (grant no. 50–2713).
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Overdiek, A., Winner, U., Mayatepek, E. et al. Schwann Cells From Human Neurofibromas Show Increased Proliferation Rates Under the Influence of Progesterone. Pediatr Res 64, 40–43 (2008). https://doi.org/10.1203/PDR.0b013e31817445b8
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DOI: https://doi.org/10.1203/PDR.0b013e31817445b8
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