Figure 5
Liver (A) and mitochondrial (B) α-TH were significantly higher in 10-day-old rats (▪) compared with adults (□); *p < 0.05 between adult and 10-day-old hepatic mitochondria. (C) α-TTP levels by immunoblot in liver homogenate were significantly increased in adult rats compared with the 10-days old. *p < 0.05 between adult and 10-day-old rat hepatic mitochondria using a student t test. In vivo α-TH administration resulted in elevated mitochondrial α-TH (D). *p < 0.05, **p < 0.005, ∥ p < 0.0005 by ANOVA analysis (E) Rats receiving a single s.c. injection of Vital E-300 (saline-•, 2.5-○, 5-▴, or 10 mg/100 g body weight-▵) exhibited an inverse curvilinear relationship between mitochondrial α-TH concentrations and mitochondrial swelling (r = −0.83, p = 0.0007) using a regression analysis. Results were from at least three separate experiments (panels A-C) or at least three animals receiving each SQ dose of α-TH.
