Figure 6
In vitro CL enrichment in mitochondria from young rats resulted in elevated concentrations of total CL (▪) and TL-CL (□). (A) Incubation of 117 nmol CL/mL mitochondria increased total CL, (p = 0.0002 for 0 vs 117 nmol CL/mL mitochondria) and TL-CL, (p = 0.0012 for 0 vs 117 nmol CL/mL mitochondria). Incubation of 11.7 nmol CL/mL mitochondria failed to significantly increase total CL or TL-CL in mitochondria. (B) Young rat mitochondria incubated with 117 nmol CL/mL mitochondria showed a small increase in bile acid-induced MPT compared with control treatment (*p ≤ 0.05). Significance was determined by ANOVA analysis. (C) Linear regression analysis revealed a positive relationship between GCDC-induced MPT and total CL concentration (r = 0.82, p < 0.001) and TL-CL concentration (r = 0.79, p < 0.001) in hepatic mitochondria of 10-day-old rats. Results were from at least four separate preparations for each CL treatment.
