Abstract
Preterm labor (PTL) is frequently associated with inflammation. We hypothesized that biomarkers during pregnancy can identify pregnancies most at risk for development of PTL. An inflammation-induced mouse model of PTL was used. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry was used to analyze and compare the plasma protein (PP) profile between CD-1 mice injected intrauterine with either lipopolysaccharide (LPS) or PBS on d 14.5 of gestation. The median differences of normalized PP peaks between the two groups were determined using the Mann-Whitney U test and the false discovery rate. In a second series of experiments, both groups of mice were injected with a lower dose of LPS. A total of 1665 peaks were detected. Thirty peaks were highly differentially expressed (p < 0.0001) between the groups. Two 11 kDa protein peaks were identified by MALDI-TOF/TOF-MS and confirmed to be mouse serum amyloid A (SAA) 1 and 2. Plasma SAA2 levels were increased in LPS-treated animals compared with controls and in LPS-treated animals that delivered preterm vs. those that delivered at term. SAA2 has the potential to be a plasma biomarker that can identify pregnancies at risk for development of PTL.
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Abbreviations
- ACN:
-
acetonitrile
- FDR:
-
false discovery rate
- IU:
-
intrauterine
- LPS:
-
lipopolysaccharide
- MW:
-
Mann-Whitney
- PTL:
-
preterm labor
- SAA:
-
serum amyloid A
- SELDI-TOF/MS:
-
surface-enhanced laser desorption/ionization time-of-flight mass spectrometry
- TFA:
-
trifluoroacetic acid
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Supported by the Hess (A.M.), Euphrates (H.J.C.), and Pediatric Research Funds (A.M.).
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Yang, Q., Whitin, J., Ling, X. et al. Plasma Biomarkers in a Mouse Model of Preterm Labor. Pediatr Res 66, 11–16 (2009). https://doi.org/10.1203/PDR.0b013e3181a207e3
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DOI: https://doi.org/10.1203/PDR.0b013e3181a207e3
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