1065 Adult Body Fat Accumulation is Not Affected by Adding Phospholipidingredient and Only Marginally By Lipid-Droplet Size in the Postnatal Diet

Abstract

Previously, we have shown that postnatal exposure to infant milk formula (IMF) with complex lipid matrix (CLM) prevented excessive diet-induced fat accumulation in adult mice (Oosting et al., 2010). Lipid droplets in CLM have physical properties closer to breast milk, i.e. large in size and coated with phospholipids (PL). We now studied the contribution of both lipid droplet size and the mere presence of dietary PL to this beneficial, protective effect of CLM on adult body composition. Postnatally, between 16 and 42 days of age, male C57Bl/6j mice were subjected to a diet containing either standard IMF (CTR; mode 0.5 µm), CLM (mode 4.3 µm), IMF with large lipid droplet size (IMF-L; mode 4.0 µm), or IMF-L with PL present in free form, not coating the lipid droplets (IMF-PL; mode 5.0 µm). Subsequently, a moderate Western style diet (10 w% fat) was fed until dissection at 98 days of age. Body composition was monitored by dual x-ray absorptiometry. Again body fat accumulation in adulthood was reduced in the CLM diet compared to the CTR diet. Increasing the dietary lipid droplet size only marginally affected body fat accumulation. Moreover, no additional effects of added PL (IMF-PL) were observed. Although increased lipid droplet size in the postnatal diet did slightly affect adult body composition, the mere presence of PL in free form had no additional effect. Evidently other aspects of CLM contribute to the observed prevention of the Western style dietinduced fat accumulation during adulthood.

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