Abstract
The novel Cav1.3 (α1D) L-type Ca2+ channel plays a significant role in sinoatrial (SA) and atrioventricular (AV) nodes function and in atrial fibrillation. However, the characterization of α1D Ca2+ channel during heart development is very limited. We used real-time RT-PCR, Western blotting, and indirect immunostaining to characterize the developmental expression and localization of α1D Ca2+ channel in rat hearts. Both protein and mRNA levels of α1D Ca2+ channel decreased postnatally. Two forms of α1D Ca2+ channel protein (250 and 190 kD) were observed, with the full-length (250 kD) channel protein being predominant in the prenatal stages. Both Western blots and confocal imaging demonstrated that α1D Ca2+ channel protein was expressed in both atria and ventricles at fetal and neonatal stages but was absent in the adult ventricles. Interestingly, α1D Ca2+ channel was also found at the nucleus/perinucleus of immature but not adult atrial cells. Furthermore, the nuclear staining was reproduced in adult atrial cell line, HL-1 cells, which possess immature properties. The data are first to show that α1D Ca2+ channel has unique age-dependent expression profile and subcellular localization in the heart, suggesting a developmental stage-dependent specific function.
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Abbreviations
- ICa-L:
-
L-type Ca2+ current
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Acknowledgements
We thank Dr. Seino and Dr. Striessnig for providing the plasmids used in this study.
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Supported by National Institutes of Health [R01 HL-077494], Veterans Affairs MERIT grants [to M.B.], and Veteran Affairs MREP grant [to Y.Q.].
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Qu, Y., Karnabi, E., Ramadan, O. et al. Perinatal and Postnatal Expression of Cav1.3 α1D Ca2+ Channel in the Rat Heart. Pediatr Res 69, 479–484 (2011). https://doi.org/10.1203/PDR.0b013e318217a0df
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DOI: https://doi.org/10.1203/PDR.0b013e318217a0df
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