Abstract
Introduction:
Air-displacement plethysmography (ADP) was developed as a noninvasive tool to assess body composition, i.e., the proportion of fat mass (%FM) and lean body mass. The results of previous studies comparing ADP with labeled water dilution in infants and with chemical analysis in phantoms have validated the ADP approach indirectly. We assessed the precision and accuracy of measurements of %FM proportions in live animals, using ADP in comparison with biochemical analyses.
Methods:
Three groups of 12 piglets each underwent four consecutive body composition assessments at 2, 7, and 21 d and were euthanized to determine whole-body lipid content by direct chemical analysis.
Results:
The average body weights were 1,490, 2,210, and 5,610 g at d2, d7, and d21, respectively. The mean %FM values determined by biochemical analysis and ADP were 8.63 ± 4.08% and 8.01 ± 4.03%, respectively. Linear regression and Bland–Altman analyses indicated good agreement for %FM. The root mean square coefficient of variation (RMS-CV) for ADP was 17.9%, with a better precision in the higher fat mass range.
Discussion:
Despite its relatively poor precision in the low range of %FM, ADP measures fat mass with reasonable precision and accuracy in the range of body weight encountered in low-birth-weight infants.
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Acknowledgements
We are grateful to Alessandro Urlando and Manoj Raghuraman for their generous help in adapting equations for application in piglets. They are employees of Life Measurement Inc. (now part of COSMED), the manufacturer of the Pea Pod Instrument. We also thank C. Tréfeu and G. Savary for their expert technical assistance. We are grateful to all the staff involved in the care of the animals.
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Frondas-Chauty, A., Louveau, I., Le Huërou-Luron, I. et al. Air-displacement plethysmography for determining body composition in neonates: validation using live piglets. Pediatr Res 72, 26–31 (2012). https://doi.org/10.1038/pr.2012.35
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DOI: https://doi.org/10.1038/pr.2012.35
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