Fig. 4

Tmem260 is expressed in murine embryos including in the heart. A–E Whole-mount in situ hybridization using mouse embryos from E8.5 to E11.5 developmental stages, and the scale bar is 500 µm. F, G Enlarged views of the heart region enclosed by the white square in (A, B), respectively. H–J Whole-mount in situ hybridization using isolated embryonic hearts. The scale bars in (F–J) are 200 µm. The panels of (E, J) show in situ hybridization with sense probe. a atrium, eh embryonic heart, fb frontal brain, fl forelimb, h heart, ha hyoid arch, hl hind limb, la left atrium, lv left ventricle, ma mandibular arch, oft outflow tract, ov otic vesicle, pa pharyngeal arch, ra right atrium, rv right ventricle, s somite