Fig. 5

The region in Tmem260 intron 3 is a putative cardiac enhancer. A In the upper panel, the structure of the mouse Tmem260 locus and flanking region with the conservation score among 100 vertebrates. A schema of a mouse 807 bp Tmem260 enhancer-lacZ reporter construct in the middle. The 807 bp region has previously-reported binding sites for multiple transcription factors with open chromatin identified using the ChIP-Atlas database. B Lateral views of representative embryos obtained with this construct are shown in the two left panels. The two pictures on the right show a magnified view of the heart. Scale bars are 500 µm. C Summary of the regions where lacZ expression was detected in F0 embryos. a atrium, fl forelimb, h head, hl hind limb, lv left ventricle, oft outflow tract, rv right ventricle, s somite, t trigeminal ganglia