Fig. 1: SOCS2 expression in p53-inducible cells.
From: Alterations in the p53-SOCS2 axis contribute to tumor growth in colon cancer
a Unsupervised hierarchical clustering separated samples into two groups: mock and p53. SOCS2 had a twofold or greater expression difference from mean at P < 0.05 based on t tests for hierarchical clustering. Data are presented in matrix format. Columns are individual cell lines and rows are genes. Red, high expression; green, low expression; black, no significant expression change between mean and sample. b Levels of SOCS2 and various p53 target proteins were analyzed by immunoblot of four p53-inducible lines (Saos, HLK3, PC3, and H1299). p53-null cells were transfected with inducible p53 plasmids (p53ERTM) and immunoblot analysis after 5 µM 4-OH tamoxifen, 24 h. c Protein (left panels) and mRNA expression levels (right panels) of SOCS2 were inversely regulated by endogenous p53 expression in HCT116 p53(+/+) cells. Dox doxorubicin
