Fig. 2: Effect of OMP deficiency on PRL synthesis in vitro. | Experimental & Molecular Medicine

Fig. 2: Effect of OMP deficiency on PRL synthesis in vitro.

From: Olfactory marker protein regulates prolactin secretion and production by modulating Ca2+ and TRH signaling in lactotrophs

Fig. 2

a PRL concentration in the supernatant of GH4 cells transfected with siCON or siOMP was evaluated using ELISA. Results represent the mean of at least three independent experiments. **P < 0.01 vs. siCON group. b RT-PCR analysis of PRL mRNA expression in GH4 cells transfected with siCON (−) or siOMP (+) (top). Semi-qRT-PCR analysis of OMP and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA levels (bottom). *P < 0.05 vs. siCON group. c Effect of siCON or siOMP on the expression of indicated proteins in GH4 cells, as determined by western blotting. d RT-PCR analysis of PRL mRNA expression in GH4 cells transfected with empty vector (−) or OMP overexpression plasmid (+) (top). Semi-qRT-PCR analysis of OMP and GAPDH mRNA levels (bottom). Data represent mean ± SE of triplicate samples. *P < 0.05 vs. empty vector group. e Expression of indicated proteins in GH4 cells transfected with empty vector of OMP overexpression plasmid, as determined by western blotting. f GH4 cells were transfected with siCON or siOMP for 48 h, and cell-cycle distribution was determined by propidium iodide staining and fluorescence-activated cell sorting. g Proliferation of GH4 cells transfected with siCON or siOMP for 5 days. Data are representative of three experiments

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