Fig. 3: Role of OMP in TRH-induced ERK1/2 phosphorylation and Ca2+ influx. | Experimental & Molecular Medicine

Fig. 3: Role of OMP in TRH-induced ERK1/2 phosphorylation and Ca2+ influx.

From: Olfactory marker protein regulates prolactin secretion and production by modulating Ca2+ and TRH signaling in lactotrophs

Fig. 3

a RT-PCR analysis of PRL mRNA expression in GH4 cells transfected with siCON or siOMP and treated with Cab (1 μM), E2 (10 nM), or TRH (100 nM). Results represent the mean of at least three independent experiments. ns, not significant; **P < 0.01 vs. non-transfected (NT) control. b PRL secretion by GH4 cells transfected with siCON or siOMP. Results represent the mean of at least three independent experiments. ns, not significant; *P < 0.05 vs. NT control. c GH4 cells were transfected with indicated siRNAs and phosphate-buffered saline (PBS) or 100 nM TRH was added for 10 min, followed by western blotting. d GH4 cells were transfected with siCON or siOMP, then loaded with Fura 2-AM and treated with TRH (100 nM) to stimulate Ca2+ release. Intracellular Ca2+ levels were measured based on ratiometric measurements of absorbance at 340 and 380 nm (340/380). Intracellular Ca2+ levels were continuously monitored for 5 min. Values represent means. e Peak store-operated Ca2+ entry in PBS-treated siCON- and siOMP-transfected cells. Values represent mean ± SE. *P < 0.05

Back to article page