Fig. 4: Inhibition of Notch1 by DAPT promotes VEGFR2 and CD31 expression in the induced membrane in the subcutaneous site and femoral defect site.
From: Inhibition of Dll4/Notch1 pathway promotes angiogenesis of Masquelet’s induced membrane in rats
a–b Protein expression of Dll4, Notch1 (NICD), Hes1, VEGFR2, and CD31 in induced membrane derived from the subcutaneous site and femoral defect site with or without DAPT intervention. c, e Immunohistochemistry staining of Notch1 (NICD) and CD31 in induced membrane derived from the subcutaneous site and femoral defect site with or without DAPT intervention (scale bar: 50 μm). d, f Quantitative results of the integral absorbance of the Notch1 and CD31-positive vessel density in induced membranes. The data in the figures represent averages ± S.D. Significant differences between the different groups are indicated as **P < 0.01, compared with saline-treated induced membrane in the subcutaneous site; ##P < 0.01, compared with saline-treated induced membrane in the femoral defect site; for each group, n = 5
