Table 1 Primer sequences used in qRT-PCR
From: Inhibition of Dll4/Notch1 pathway promotes angiogenesis of Masquelet’s induced membrane in rats
Gene | Forward primer | Reverse primer |
|---|---|---|
DLL1 | 5′- CACGAGAAAACCAGAAAGC-3′ | 5′- ATGCCCGGAAAGTCTATGTG-3′ |
DLL3 | 5′- ACTGGATGCCTTTTACCTGG -3′ | 5′-ACATCGAAGCCCGTAGAATC-3′ |
DLL4 | 5′- CTTTACCGGCTCTAACTGTGAG-3′ | 5′-GGCTTGGACCTCTGTTCAG-3′ |
Jagged1 | 5′- GGTGGACAGCTCTGTGACAA-3′ | 5′-CAGCCTGGAGAACACTCACA-3′ |
Jagged2 | 5′-ACGAGGAGGATGAAGAGCTGA-3′ | 5′-GGGGTCTTTGGTGAACTTGTG-3′ |
Notch1 | 5′- TGAGACTGCCAAAGTGTTGC-3′ | 5′- GTGGGAGACAGAGTGGGTGT-3′ |
Notch2 | 5′-CCTGAACGGGCAGTACATTT-3′ | 5′-GCGTAGCCCTTCAGACACTC-3′ |
Notch3 | 5′-ATACACTGGCCCCTTCTGTG-3′ | 5′-GTCGAGGCAAGAACAGGAAA-3′ |
Notch4 | 5′-CTCTGTCCGCCTTCTTTCTG-3′ | 5′-CAAGCACACACCTCCGTTG-3′ |
β-actin | 5′- AGCCATGTACGTAGCCATCC-3′ | 5′- GCTGTGGTGGTGAAGCTGTA-3′ |
