Fig. 3: A/E inactivates BASP1 through direct promoter binding. | Experimental & Molecular Medicine

Fig. 3: A/E inactivates BASP1 through direct promoter binding.

From: Methylation-associated silencing of BASP1 contributes to leukemogenesis in t(8;21) acute myeloid leukemia

Fig. 3

a The left diagram shows a luciferase reporter containing various lengths of the BASP1 promoter region with wild-type A/E-binding sites1,2,3,4 or mutated sequence (mut). Luciferase reporter assays showed that A/E repressed the transcriptional activity of the BASP1 promoter. b ChIP was performed in the SKNO-1 and SKNO-1-siA/E cell lines using anti-AML1, anti-ETO, anti-DNMT1, anti-DNMT3a, and anti-DNMT3b antibodies or a non-specific antibody (IgG control). Quantitative RT-PCR was performed with primers specific for the BASP1 promoter region surrounding the predicted −546/−556 binding site and expression was quantified as 2−ΔCT relative to input. ChIP-PCR showed that A/E and DNMT3a bound the BASP1 promoter (*p < 0.05, **p < 0.01)

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