Table 1 Comparison of scRNA-seq library preparation methods

From: Single-cell RNA sequencing technologies and bioinformatics pipelines

Platform

Smart-seq

MARS-seq

CEL-seq

Drop-seq

Region

Full-length

3′ end

3′ end

3′ end

Target read depth (per cell)

(106)

(104)–(105)

(104)–(105)

(104)–(105)

UMI

None

Yes

Yes

Yes

Amplification

PCR

IVT

IVT

PCR

Feature

Isoform analysis

FACS sorting Multiplex barcoding

Linear amplification (pool cDNAs for IVT)

Emulsion Low cost

  1. scRNA single-cell RNA sequencing, Smart-seq novel full-transcriptome mRNA-sequencing protocol, CEL-seq cell expression by linear amplification and sequencing, Drop-seq droplet sequencing, IVTin vitro transcription, UMIunique molecular identifier, FACSflow-activated cell sorting, MARS-seqmassively parallel RNA single-cell sequencing framework
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