Fig. 5: Knockdown of Cldn11 suppresses OB differentiation.
From: Claudin 11 regulates bone homeostasis via bidirectional EphB4-EphrinB2 signaling
a OBs transfected with siControl or siCldn11 were cultured for 7 days in osteoblastogenic medium. ALP-positive cells were monitored by ALP staining, and ALP activity was expressed as a function of released p-nitrophenol. b Calcium deposits for matrix mineralization were measured by ARS staining after 21 days, and the intensity of staining was quantified with CPC. c Total cell lysates from OBs transfected with siControl or siCldn11 were analyzed by carrying out western blotting with the indicated antibodies. β-actin was used as an internal control. Data are presented as the mean ± SD of three independent experiments. **P < 0.01; ***P < 0.001 vs. the siControl
