Fig. 2: Gastrin regulates the focal adhesion (FA) formation, F-actin polarization, and migration of PANC-1 cells through the Gα12 pathway. | Experimental & Molecular Medicine

Fig. 2: Gastrin regulates the focal adhesion (FA) formation, F-actin polarization, and migration of PANC-1 cells through the Gα12 pathway.

From: Gastrin stimulates pancreatic cancer cell directional migration by activating the Gα12/13–RhoA–ROCK signaling pathway

Fig. 2

PANC-1 cells were treated with control or Gα12 siRNA as indicated. a As indicated by immunoprecipitation experiments, RNAi-mediated Gα12 protein depletion significantly suppressed the phosphorylation of paxillin activated by gastrin in PANC-1 cells. b RNAi-mediated Gα12 protein depletion also restrained PANC-1 cells from the elevated GTP-RhoA level that was activated by gastrin. c, d Focal adhesion formation and F-actin polarization were evaluated by immunofluorescence (c) and were represented by the average number of focal adhesions that were labeled by paxillin per cell (d). White arrows show the focal adhesion formation and aggregation at the leading edge of the cells. e, f The scratched wound healing assay (e) and Boyden invasion assay (f) were conducted to show the migration and invasion of PANC-1 cells. The average number of focal adhesions from a minimum of 20 cells was quantified. The other values indicate the means ± SD from three independent experiments. *P < 0.05

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