Fig. 4: Gastrin-regulated PANC-1 cell polarization, migration, and invasion through activation of ROCK. | Experimental & Molecular Medicine

Fig. 4: Gastrin-regulated PANC-1 cell polarization, migration, and invasion through activation of ROCK.

From: Gastrin stimulates pancreatic cancer cell directional migration by activating the Gα12/13–RhoA–ROCK signaling pathway

Fig. 4

PANC-1 cancer cells were pre-incubated with Y27632 reagent, an effective inhibitor of ROCK, for 4 h and were then stimulated with gastrin for 1 h. a Activated paxillin with high levels of phosphorylation at Tyr118 and Tyr31 that were induced by gastrin were significantly inhibited by Y27632 pre-treatment, while total paxillin was unchanged. b Immunofluorescence images indicate an increased polarization of F-actin and aggregation of focal adhesions at the leading edge in gastrin-treated PANC-1 cells. Y27632 pre-treatment obviously blocked the focal adhesion enrichment and cell polarization. Boxed regions represent the area of the merged image used for the zoom; c The mean number of large-sized focal adhesions labeled by paxillin per cell are indicated, and the average number of focal adhesions from a minimum of 20 cells was calculated. d, e The migration and invasion of PANC-1 cells pre-treated with Y27632 were investigated by scratched wound healing (d) and the Boyden invasion assay (e). Values indicate the means ± SD from three independent experiments. *P < 0.05

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