Fig. 1: NDRG3 suppresses the hypoxic induction of HIF-1/2α proteins.

a PC3 and DU145 cells were incubated under hypoxia (1% O₂) for the indicated times and subjected to western blotting with an anti-HIF-1α or anti-HIF-2α antibody. b PC3 and DU145 cells, which had been transfected with siRNAs silencing HIF-1α or HIF-2α, were incubated under normoxia or hypoxia for 24 h and subjected to western blotting. c PC3 and DU145 cells, which had been transfected with NDRG3-silencing siRNA (siNDRG3 #1), were incubated under normoxia or hypoxia for 24 h and subjected to western blotting. d PC3 and DU145 cells, which had been transfected with NDRG3-silencing siRNA, were incubated under normoxia or hypoxia for 24 h and lysed for RNA extraction. PDK1 and BNIP3 mRNA levels were measured by RT-qPCR. Each bar represents the mean + s.d. (n = 3) and * denotes P < 0.05 between the indicated groups