Fig. 6: NDRG3 inhibits the migration and invasion of prostate cancer cells. | Experimental & Molecular Medicine

Fig. 6: NDRG3 inhibits the migration and invasion of prostate cancer cells.

From: NDRG3 lowers the metastatic potential in prostate cancer as a feedback controller of hypoxia-inducible factors

Fig. 6

PC3 (a, c) or DU145 (b, d) cells were transfected with siNDRG3 or MYC-NDRG3, and 1 × 104 cells were seeded in a transwell culture plate. The upper chambers were filled with serum-free media and the lower chambers with 10% FBS-containing media. Migration and invasion analyses were performed using uncoated and Matrigel-coated interface membranes, respectively. After cells were incubated under normoxia or hypoxia for 24 h, the membranes were fixed in 4% PFA and subjected to H&E staining. Representative images of each group are shown on the left, and the numbers (means + s.d., n = 3) of migrated or invaded cells are shown as bar graphs on the right. * denotes P < 0.05

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