Fig. 7: NDRG3 expression inversely correlates with prostate cancer progression and HIF-1α expression.

a Relative NDRG3 mRNA expression in normal adjacent to tumor tissues (NAT, n = 59), primary tumor tissues (PT, n = 66), and metastatic tumor tissues (MT, n = 25), which were obtained from GEO data set GSE6919. b The GSE6919 data set is divided into NDRG3_Low and NDRG3_High groups according to the median value (961.25) of the NDRG3 mRNA level. c The GSEA enrichment plot for the PID_HIF1_TFPATHWAY gene set in the NDRG3_Low group. d IHC analyses of NDRG3 and HIF-1α protein expressions in a human prostate adenocarcinoma tissue microarray. Representative images are shown on the left and dot plots of the immunostaining scores are shown on the right. The scale bar represents 50 μm. e Kaplan–Meier analysis for the association of NDRG3 or HIF-1α expression and the tumor-free survival rates in the human prostate adenocarcinoma tissue microarray. The Low and High groups were categorized according to the median values of the protein expressions. P-value was calculated by log-rank test. f Pearson correlation analysis shows a negative correlation between NDRG3 and HIF-1α expression in the tissue microarray. R value indicates the Pearson correlation coefficient value. The horizontal lines in all dot plots represent the means ± s.e.m. and * denotes P < 0.05 between the indicated groups