Table 2 Summary of NAA10 mutations in organisms
From: N-α-acetyltransferase 10 (NAA10) in development: the role of NAA10
Organism scientific name | Homolog | Symbol of mutation | Type of mutation | Protein effect/ molecular mechanism | Phenotype | Ref. |
|---|---|---|---|---|---|---|
Yeast Saccharomyces cerevisiae | ARD1, yNaa10 | ard1::HIS3 | Inserting a Barn HI fragment containinng the HIS3 gene into the Barn HI site of plasmid YCpE18 that lies within the functional sequence of ARD1 | Defect in transcription of a-specific genes, Permit expression of the information resident at HML | Reduced viability, sensitive to heat shock and salt, fail to enter stationay phase, lack of glycogen accumulation, sporulation defect, poor mating, fail to undergo meiosis | |
nat1-5::LEU; ard1 | Mating nat1 and ard1 single mutant for nat1 ard1 double mutant | Impaired N-terminal activity (predicted); single mutants of nat1 (Naa15) and ard1 (Naa10) displayed identical phenotypes, no additional phenotypes are found in double mutant | Inable to sporulate, slow growth, reduced mating, inhibit sporulation, impaired resistance to heat shock, fail to G1 arrest, partial depression of HML, fail to accumulate storage | |||
y[hNatA S37P] | A strain without yNatA and expression human NatA with a mutated hNaa10 S37P | Lack of proper complex formation with hNaa15 and reduced in vitro catalytic activity, decreased of Nt-acetylome using COFRADIC, increase in the Hsp70 family proteins | Growth defect, sensitive to caffeine and cycloheximide, Impaired resistance to heatshock, decreased mating efficiency | |||
yS39P | S39P mutation in homologous position to human Naa10 S37 | No obious effects | ||||
Plant Arabidopsis | At5g13780, AtNAA10 | naa10-1 | T-DNA insertion-disrupting gene expression | Impaired N-terminal activity; Naa15 mutation also shared same phenotypes | Growth retardation in vegetative stage, lethal, abortion of embryogenesis, drought-adapted root morphology | |
amiNaa10 | Depleted-RNA silencing | |||||
Protozoan parasite Trypanosoma brucei | TbARD1 | Ard1 null mutant | Removal of the ARD1 coding region | Impaired N-terminal activity (predicted) | Impaired growth in bloodstream-form cells, reduced differentiation to insect-stage cells | |
Worm Caenorhabditis elegans | DAF-31 | daf-31(m655) | Remove 151 bp of promoter upstream of the ATG start codon and 242 bp of daf-31 coding region dowstream of the ATG start codon | Regulates the transcriptional activity of DAF-16 (FOXO transcription factor) | Developmental larval arrest, fat accumulation, formation of dauer-like larvae under starvation conditions, decreased lifespan, no SDS-resistant, cannot resume development and reproduction after food re-providing | |
daf-31 RNAi | RNAi knock down; reducing daf-31 mRNA | Decreased lifespan | ||||
daf-31 OE | Overexpression; full length dar-31 genomic DNA was cloned into pGEM-T | Increase lifespan in daf-2 mutant, enhancing reproduction | ||||
Fruit fly Drosophila melanogaster | vnc | vncBDk | Frame shift mutation in acetyltransferase-truncated enzymatic region | Impaired N-terminal activity (predicted) | Pleiotropic oogenesis, aberrant mitosis, egg chamber encapsulation defects, nurse cell chromatin dispersion defects | |
vnc14 | Copia insertion in the intron | |||||
Zebra fish Danio rerio | wufc66b08, zNaa10 | naa10MO | Morpholino-based knockdown | Predicted N-terminal activity; zNaa10 has identical substrate specificity to hNaa10 in vitro | Lethality, growth retardation, bent axis and tails, abnormal eyes, less pigmentation |
