Fig. 3: Cellular processes represented by DSPs between oleate-treated and untreated samples and network models delineating key cellular processes.

a, b Cellular processes (GOBPs) enriched by upregulated (a) and downregulated (b) secreted proteins between oleate-treated and untreated samples. Each bar represents the Z-score of the P value that is the significance of the corresponding GOBP being enriched by the DSPs. The dashed line denotes the cutoff of P = 0.01. Bar colors represent the groups of GOBPs associated with the indicated cellular events. c–e Network models describing interactions among DSPs involved in FA removal and production (c), inflammatory responses and cell adhesion/migration (d), and ER stress and protein degradation (e). Node colors represent up- (red) and down-regulation (green) of secreted proteins after oleate treatment. Large nodes indicate the six secreted proteins selected as a secretome profile in this study. Nodes were arranged according to GOCCs and the localization information obtained from the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database. Gray lines represent protein-protein interactions, and the arrows and inhibition symbols obtained from the KEGG pathway database denote activation and suppression, respectively. Thick black lines denote membranes, such as plasma membrane (PM), lysosomal (c) and ER (e) membranes, and vesicular membranes (c)