Fig. 4: Validation of upregulation and downregulation of the selected secreted proteins after oleate treatment. | Experimental & Molecular Medicine

Fig. 4: Validation of upregulation and downregulation of the selected secreted proteins after oleate treatment.

From: A secretome profile indicative of oleate-induced proliferation of HepG2 hepatocellular carcinoma cells

Fig. 4

a–f Western blotting analysis of the six selected secreted proteins using an independent set of the conditioned media samples (n ≥ 4): MIF (a), THBS1 (b), PDIA3 (c), APOA1 (d), EEF2 (e), and FASN (f). The results showed significant (P < 0.05) upregulation of MIF, THBS1, PDIA3, and APOA1 and downregulation of EEF2 and FASN in oleate-treated samples compared to the untreated controls. The data are shown as the mean ± SEM. *P < 0.05 by Student’s t-test

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