Fig. 3: CircRNA 104670 serves as a sponge for the miR-17-3p and MMP-2 is directly targeted by miR-17-3p. | Experimental & Molecular Medicine

Fig. 3: CircRNA 104670 serves as a sponge for the miR-17-3p and MMP-2 is directly targeted by miR-17-3p.

From: CircularRNA_104670 plays a critical role in intervertebral disc degeneration by functioning as a ceRNA

Fig. 3

a The binding region of miRNAs in circRNA_104670 3′UTR are shown. b A luciferase reporter assay confirmed that miR-17-3p, miR-22-5p, miR-96-3p, and miR-433-3p reduce the luciferase intensity by more than 40%. c, d A pmiR-RB-Report vector with or without the 3′UTR sequence of circRNA_104670 was co-transfected with miR-17-3p or a negative control oligonucleotide; RLU of hRluc and hLuc+ were constructed, and the hRluc values were normalized to the corresponding hLuc+ values, **p < 0.01. e, f NP cells were co-transfected with an EGFP reporter plasmid (containing 3′UTR of MMP-2) or the mutant vector (containing mutant 3′UTR of MMP-2) the pDsRed-C1 plasmid either alone or in combination with a miR-17-3p mimic. An F-4500 fluorescence spectrophotometer was used to analyze the EGFP and RFP levels. Scale bar, 40 μm, **p < 0.01

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