Fig. 1: The expression level of PRMT1 increased in RANKL-treated bone marrow-derived macrophages (BMDMs).
From: PRMT1 mediates RANKL-induced osteoclastogenesis and contributes to bone loss in ovariectomized mice
a-d BMDMs were cultured with M-CSF (30 ng/ml) and RANKL (100 ng/ml) for the indicated time periods to induce differentiation into mature osteoclasts. a Cellular proteins were extracted and subjected to western blotting with the indicated antibodies. b Results of real-time PCR showing the mRNA expression level of PRMT1. The results were normalized to the expression level of β-actin. c Expression level of ADMA in a culture supernatant determined by ELISA. The results are expressed as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. 0 d. d BMDMs were labeled with anti-PRMT1 and a fluorescein isothiocyanate (FITC)-conjugated secondary antibody. F-actin ring formation was stained with Alexa Fluor 594-phalloidin and detected using a confocal microscope. The representative images are from at least three independent experiments. Scale bar: 5 μm
