Fig. 3: GRP78 expression in the PVN of ob/ob and diet-induced obese mice. | Experimental & Molecular Medicine

Fig. 3: GRP78 expression in the PVN of ob/ob and diet-induced obese mice.

From: Glucose-regulated protein 78 binds to and regulates the melanocortin-4 receptor

Fig. 3

Sections were prepared from the obese mouse models: 10–12-week-old ob/ob male mice and high-fat diet (HFD)-fed DIO mice. WT and normal diet (ND)-fed male mice were used as controls. Representative coronal sections (40 µm) stained with an antibody against GRP78 and visualized by 3,3ʹ-diaminobenzidine reactions are shown. a GRP78 expression in the hypothalamic PVN region. The inset in the panel shows the high-magnification area of the PVN within the low-magnification image (scale bar: low: 250 µm, high: 100 µm). b Representative images of GRP78 staining of sections from the PVN of ob/ob and WT mice (scale bar, 100 µm). c Representative GRP78 staining images in the PVN region of DIO mice (HFD) and control mice (ND) (scale bar, 100 µm). The number of GRP78-positive cells in the PVN was counted using stereological analysis, and the graph shows the percentage of the average total number of neurons/nucleus/WT or ND mice. One-tailed Student’s t test for b: n = 4 per group, *P < 0.05 versus WT mice; One-tailed Student’s t test for c: n = 4–5 per group, **P < 0.01 versus ND-fed mice. The data are presented as the mean ± SEM

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