Fig. 5: Physiological effects of lentivirus-mediated GRP78 knockdown in the PVN.
From: Glucose-regulated protein 78 binds to and regulates the melanocortin-4 receptor

Lenti-shGRP78 or Lenti-control viruses were stereotaxically delivered to the PVN of 6-week-old male mice. After surgery and recovery (for 7 days), mice were fed an ND or HFD for 40 days. Body weight and food intake were measured daily at 5:00 p.m. Before killing, oxygen consumption (for 24 h) and locomotor activity (for 1 h) were measured. a Schematic of the lentiviral vector-encoding GRP78 short hairpin RNA (top), and the injection sites in the PVN shown in a coronal diagram of the brain (bottom) (scale bar, 100 µm). Real-time PCR analysis of GRP78 mRNA in the PVN. Changes in b body weight, c food intake, d oxygen consumption, and e locomotor activity in mice fed an ND or HFD. One-tailed Student’s t test for a: n = 3 per group, **P < 0.01 versus Lenti-control. Two-way ANOVA, Bonferroni post hoc tests for b–e: n = 3–4, #P < 0.05, ##P < 0.01, ###P < 0.001 versus ND, *P < 0.01 versus Lenti-control. The data are presented as the mean ± SEM