Fig. 5: HS-1371 had an inhibitory effect on RIP3 kinase activity in various cell lines. | Experimental & Molecular Medicine

Fig. 5: HS-1371 had an inhibitory effect on RIP3 kinase activity in various cell lines.

From: HS-1371, a novel kinase inhibitor of RIP3-mediated necroptosis

Fig. 5

a, b HeLa (cervical cancer, a) and H2009 (lung cancer, b) cells ectopically expressing RIP3 were treated with indicated concentrations of HS-1371 for 6 h, and cell lysates were analyzed by immunoblotting (left upper panel). Cells were pretreated with DAB or HS-1371 for 2 h and then treated with TSZ (6 h for immunoblotting, 24 h for cell death assay). Cell lysates were analyzed by immunoblotting (left bottom panel), and cell viability was analyzed by MTT assay (right panel). The results are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. c, d HS-1371 had the same effect on mouse cells. L929 (mouse sarcoma, c) and MEF (mouse embryonic fibroblast, d) cells were pretreated with inhibitors and then necroptosis was induced by TNF. Cell lysates were analyzed by immunoblotting (upper panel), and cell viability was analyzed by MTT assay (bottom panel). The results are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

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