Fig. 6: Possibility of HS-1371 as a drug for necroptosis-related diseases. | Experimental & Molecular Medicine

Fig. 6: Possibility of HS-1371 as a drug for necroptosis-related diseases.

From: HS-1371, a novel kinase inhibitor of RIP3-mediated necroptosis

Fig. 6

a, b HT-29 cells were pre- or post-treated with HS-1371 for indicated time points, and RIP3 phosphorylation (a) and cell death (b) were measured by western blotting or MTT assay. Data are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. c HT-29 cells were treated with TSZ and then post-treated with HS-1371 for indicated time points, and then cell lysates were analyzed by immunoblotting (upper panel). Cell viability was analyzed by MTT assay. Data are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. d Cells were treated with the same conditions as (c), and cell lysates were immunoprecipitated with anti-RIP3 antibody. Immunoprecipitates and total lysates were analyzed by Western blotting. e Upregulated RIP3 expression-induced cell death was inhibited by treatment with HS-1371. 293T cells were transfected with Flag vector or Flag-RIP3, and 20 h after transfection, cells were stained with PI. PI-positive cells were analyzed by phase-contrast fluorescence microscopy

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