Fig. 2: The miR-92a is an oncogenic miRNA that plays critical roles in mediating ERβ-promoted BCa cell growth and invasion.

a Real-time PCR shows miR-92a levels in J82 cells transduced with lentiviral ERβ shRNA (shERβ) with/without miR-92a or control (Ctrl) and in UMUC3 cells overexpressed with lentiviral ERβ cDNA (oeERβ) with/without miR-92a inhibitor. We first showed in the J82 cells (left) that shERβ can reduce miR-92a expression. In UMUC3 cells (right), when ERβ is overexpressed, the miR-92a was up-regulated. The transfection of miR-92a inhibitor (oligonucleotide) can significantly reduce ERβ-upregulated miR-92 in UMUC3 cells. b J82 cells were prepared as in A for MTT assays. Ectopic miR-92a expression could reverse the shERβ-reduced BCa growth in J82 cells. Analysis of cell growth was conducted by MTT assay on days 0, 2, 4 and 6. c UMUC3 cells were prepared as described in A. The miRNA-92a inhibitor could dramatically reduce ERβ-promoted cell growth in these UMUC3 cells. d Ectopic miR-92a expression could reverse shERβ-reduced BCa invasion in J82 cells. Invasion assays were conducted using the transwell Matrigel invasion assay. e Transfection of UMUC3 cells with miR-92a inhibitor can reduce ERβ-promoted cell invasion. Data are represented as the mean ± SD