Fig. 3: CLOCK and BMAL1 upregulate the expression of RHOA protein.
From: CLOCK and BMAL1 stabilize and activate RHOA to promote F-actin formation in cancer cells
a Immunofluorescent staining of RHOA (Red) in BMAL1+, control, and CLOCK+ HeLa cells (left panel). Nuclei were stained with DAPI. The scale bars represent 10 μm. The quantification of RHOA was performed with ImageJ and analyzed (right panel) **p < 0.01. b, c Expression of RHOA at the protein level was examined using western blotting in shBMAL1, BMAL1+, shCLOCK, and CLOCK+ HeLa cells. RHOA expression was quantified with ImageJ and then normalized to GAPDH expression. The protein level of the control was taken as 1. Data were analyzed using a t test (n = 3) and presented as the mean ± sd; *p < 0.05; **p < 0.01. d Expression of RHOA at the mRNA level was examined by qPCR in shBMAL1, BMAL1+, shCLOCK, and CLOCK+ cells. Data are presented as the mean ± sd (n = 3); n.s. not significant
