Fig. 6: CLOCK and BMAL1 enhance F-actin filaments via the RHOA-ROCK-CFL pathway.
From: CLOCK and BMAL1 stabilize and activate RHOA to promote F-actin formation in cancer cells
a RHOA abundance was verified by western blotting in RHOA overexpression (RHOA+), RHOA knockdown (shRHOA), and control HeLa cells. GAPDH was used as a loading control. Data are presented as the mean ± sd (n = 3); *p < 0.05; **p < 0.01. b, c F-actin was stained with TRITC-phalloidin (Red) in HepG2 cells transfected with vectors. Nuclei were stained with DAPI. The scale bars represent 10 μm. The F-actin levels were quantified with the fluorescence intensity ratio using ImageJ. The fluorescence intensity of the control was taken as 1. Data were analyzed using a t test (n = 3) and presented as the mean ± sd; *p < 0.05; **p < 0.01. d F-actin and G-actin from HeLa cells were segmented by ultraspeed centrifugation and analyzed by western blotting using an antibody against β-actin. The data were quantified using ImageJ. *p < 0.05; **p < 0.01, n.s. not significant
