Fig. 7: CLOCK and BMAL1 promote cell proliferation, migration, and invasion.
From: CLOCK and BMAL1 stabilize and activate RHOA to promote F-actin formation in cancer cells
a The cell viability of HeLa cells was determined by Cell Counting Kit-8 assay after plasmid transfection. The results were normalized to the control group (mean ± SD, n = 3). b Representative pictures of the scratch wound healing assay. During a 48-h wound closure assay, pictures were taken every 24 h using a time-lapse microscope. Representative pictures at 0, 24, and 48 h are shown. The distance of the wound was measured, and two line charts were used to display the width of the wound over time. Data were analyzed using a t test (n = 3) and presented as the mean ± sd. c Representative microscopic images of cells that migrated through the transwell in the migration assay. After cell migration and staining with crystal violet, pictures of the migrated cells (purple stained) were taken using a microscope. **p < 0.01
