Fig. 3: Coactivator function of MSK1 in p53-mediated in vitro transcription.
From: MSK1 functions as a transcriptional coactivator of p53 in the regulation of p21 gene expression
a SDS-PAGE/Coomassie blue staining and immunoblot analyses with the indicated antibodies for purified FLAG-MSK1 from HEK293T cells in the absence (MSK1, lane 1) and presence (MSK1*, lane 2) of MKK6ca co-expression. b Effects of MSK1 in p53-mediated in vitro transcription of a chromatin template. Standard p53- and p300-dependent transcription from a chromatinized p53ML plasmid was performed according to the scheme in the top panel. Reactions contained 5 ng (lanes 5 and 7) or 20 ng (lanes 6 and 8) of MSK1 where indicated. c Direct effects of histone H3 phosphorylation in the p53-mediated in vitro transcription of a chromatin template. In vitro transcription assays were performed with chromatin templates assembled with recombinant histone octamers containing fully phosphorylated H3S10 or H3S28. Relative transcription levels were measured using a phosphoimager and normalized to that of p53 and p300 addition (b and c, lane 4)
