Fig. 5: Characterization of the phosphorylation status of MSK1 phosphomimetic mutants.
From: MSK1 functions as a transcriptional coactivator of p53 in the regulation of p21 gene expression
a–c The phosphorylation status in the activation loops of purified wild-type and phosphomimetic MSK1 with a single (a), double (b), and triple (c) number of mutant fragments was monitored by immunoblotting with anti-S212 and anti-T581 phospho-specific antibodies. MSK1 and MSK1* wild-type MSK1 were prepared in the absence and presence of MKK6ca co-expression, respectively. The data are representative of at least three independent experiments. MSK1 phosphomimetic mutants with active histone kinase activity (Fig. 4) are indicated by underlining
